Solubilization and partial purification of squalene synthase from daffodil microsomal membranes
- 4 November 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 292 (1-2) , 34-36
- https://doi.org/10.1016/0014-5793(91)80827-p
Abstract
A squalene synthase was solubilized from daffodil (Narcissus pseudonarcissus L.) microsomes with CHAPS, a zwitterionic non-denaturating detergent. By successive chromatography on DEAE Sephacel and APP Sepharose a fraction enriched in this enzyme (21-fold) was preparedKeywords
This publication has 14 references indexed in Scilit:
- Partial purification and characterization of two sesquiterpene cyclases from sage (Salvia officinalis) which catalyze the respective conversion of farnesyl pyrophosphate to humulene and caryophylleneArchives of Biochemistry and Biophysics, 1988
- Isolation by an Aqueous Two-Polymer Phase System and Identification of Endomembranes from Citrofortunella mitis Fruits for Sesquiterpene Hydrocarbon SynthesisJournal of Plant Physiology, 1988
- Purification to homogeneity and some properties of squalene synthetaseArchives of Biochemistry and Biophysics, 1988
- Purification of isopentenyl pyrophosphate isomerase and geranylgeranyl pyrophosphate synthase from Capsicum chromoplasts by affinity chromatographyBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987
- Music Supervisor/AdministratorMusic Educators Journal, 1982
- The site of carotenogenic enzymes in chromoplasts from Narcissus pseudonarcissus L.Planta, 1982
- A nondenaturing zwitterionic detergent for membrane biochemistry: design and synthesis.Proceedings of the National Academy of Sciences, 1980
- Squalene synthetase. Solubilization from yeast microsomes of a phospholipid-requiring enzymeJournal of Biological Chemistry, 1978
- A simplified method for the quantitative assay of small amounts of protein in biologic materialAnalytical Biochemistry, 1973
- Genetics The Science of Genetics G. W. BurnsBioScience, 1969