Induction of murine macrophage TNF-α synthesis byMycobacterium aviumis modulated through complement-dependent interaction via complement receptors 3 and 4 in relation toM. aviumglycopeptidolipid
Open Access
- 1 May 2005
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 246 (2) , 221-228
- https://doi.org/10.1016/j.femsle.2005.04.008
Abstract
We studied whether complement receptor (CR) mediated Mycobacterium avium interaction modulated macrophage TNF-α expression. Compared to control conditions, infections performed with C3-depletion yielded significantly higher TNF-α levels. Blockage of the CR4 iC3b site yielded increases in TNF-α for all morphotypic variants of a virulent serovar-8 strain (smooth transparent (SmT), smooth opaque (SmO), serovar-specific glycopeptidolipid (ssGPL) deficient knockout mutant) whereas CR3 blockage increased TNF-α only for SmT and ssGPL-deficient strains. Thus, complement-mediated binding of M. avium to CR3 and CR4 was shown to modulate TNF-α expression. The differential activation of morphotypic and isogenic variants of a single strain provides an excellent model system to delineate signaling pathways.Keywords
This publication has 20 references indexed in Scilit:
- Macrophage—Mycobacterium tuberculosis interactions: role of complement receptor 3Microbial Pathogenesis, 2003
- Gram-negative bacteria and phagocytic cell interaction mediated by complement receptor 3FEMS Immunology & Medical Microbiology, 2002
- Differential Responses of Bovine Macrophages toMycobacterium aviumsubsp.paratuberculosisandMycobacterium aviumsubsp.aviumInfection and Immunity, 2002
- Control of Mycobacterial Replication in Human Macrophages: Roles of Extracellular Signal-Regulated Kinases 1 and 2 and p38 Mitogen-Activated Protein Kinase PathwaysInfection and Immunity, 2002
- Mycobacteria-Induced TNF-α and IL-10 Formation by Human Macrophages Is Differentially Regulated at the Level of Mitogen-Activated Protein Kinase ActivityThe Journal of Immunology, 2001
- Lethal Granuloma Disintegration in Mycobacteria-Infected TNFRp55−/− Mice Is Dependent on T Cells and IL-12The Journal of Immunology, 2000
- T‐cell‐independent granuloma formation in response to Mycobacterium avium: role of tumour necrosis factor‐α and interferon‐γImmunology, 1997
- A Macrophage Invasion Mechanism of Pathogenic MycobacteriaScience, 1997
- Tumour necrosis factor-alpha (TNF-α) in the host resistance to mycobacteria of distinct virulenceClinical and Experimental Immunology, 1995
- Receptors for C3b and C3bi promote phagocytosis but not the release of toxic oxygen from human phagocytes.The Journal of Experimental Medicine, 1983