Isolation of Fibronectin under Mild Conditions
- 30 June 1984
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 47 (1) , 41-46
- https://doi.org/10.1111/j.1423-0410.1984.tb01559.x
Abstract
Fibronectim was isolated from human blood plasma by affinity chromatography on immobilized Physiogel, a plasma expander made by chemical degradation of gelatin. Binding of fibronectin to Physiogel is weaker than to gelatin; elution could therefore be performed under rather mild conditions: at pH 6.5 and 30.degree. C. Loading of the sample at low temperature increases the capacity of the affinity column.This publication has 15 references indexed in Scilit:
- FibronectinArteriosclerosis, Thrombosis, and Vascular Biology, 1998
- Hydrophobic chromatography of fibronectinFEBS Letters, 1982
- Rapid methods for isolation of human plasma fibronectinThrombosis Research, 1982
- A novel method for purification of plasma fibronectinBiochimica et Biophysica Acta (BBA) - General Subjects, 1982
- Interaction of fibronectin with collagen fibrilsBiochemistry, 1981
- Fibronectins—adhesive glycoproteins of cell surface and bloodNature, 1978
- New methods for the preparation of biospecific adsorbents and immobilized enzymes utilizing trichloro-s-triazineAnalytical Biochemistry, 1978
- Binding of soluble form of fibroblast surface protein, fibronectin, to collagenInternational Journal of Cancer, 1977
- A simplified method for cyanogen bromide activation of agarose for affinity chromatographyAnalytical Biochemistry, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970