THE NATURE OF ADRENALINE AND NORADRENALINE IN NORMAL HUMAN URINE*

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Abstract
Studies were conducted to determine the nature of epinephrine (A) and norepinephrine (NA) present in normal human urine. In addition to acid hydrolysis, the urine was incubated with [beta]-glucuronidase and with Mylase P as a source of phenolsulfatase. With acid hydrolysis, the highest values of NA were obtained after 5 minutes of boiling at pH 1.5. Definite loss of NA and A activity was seen after boiling for periods of 15 to 30 minutes. NA is conjugated to the extent of 60%, whereas A is in all likelihood free. The conjugate of NA in normal urine appears to be the glucuronide and not the sulfate.