Terminal Autoreceptor Control of 5‐Hydroxytryptamine Release as Measured by In Vivo Microdialysis in the Conscious Guinea‐Pig

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Abstract
In vivo microdialysis in the frontal cortex of the freely moving guinea‐pig was used to measure extracellular 5‐hydroxytryptamine (5‐HT) and study terminal autoreceptor control of its release. The indoleamine levels were determined by HPLC with electrochemical detection. Release of extracellular 5‐HT and, to a lesser extent, 5‐hydroxyindoleacetic acid was sensitive to tetrodotoxin, confirming the neuronal origin of measured neurotransmitter levels. Both systemic and local administration of the 5‐HT1 agonist 5‐carboxamidotryptamine caused inhibition of extracellular 5‐HT levels, confirming the regulatory role of the terminal, and possibly also the somatodendritic, 5‐HT autoreceptor on neuronal 5‐HT release. Levels of extracellular 5‐hydroxyindoleacetic acid were not affected by 5‐carboxamidotryptamine following either central or peripheral administration.

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