Plasmid-controlled resistance to copper in Escherichia coli

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Abstract
The Cu resistance of a strain of E. coli isolated from the effluent of a piggery where pigs were fed a diet supplemented with CuSO4 was controlled by a conjugative 78-megadalton plasmid designated pRJ1004. Plasmid pRJ1004 exhibited surface exclusion and incompatibility with standard plasmids belonging to incompatibility groups I1 and K. Sensitive strains of E. coli K-12 were unable to form colonies on nutrient agar containing > 4 mM Cu; transconjugants which harbored pRJ1004 were able to form colonies on medium containing up to 20 mM Cu.