Growth on amino acids, cell wall proteins, and hydrolysis of yeast phase cell walls ofHistoplasma capsulatumandH. duboisii

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Abstract
Unlike Histoplasma capsulatum, all strains of H. duboisii were stimulated by tyrosine or proline, giving shorter lag-periods and increased cell yields when grown on a basal medium containing cysteine, aspartic acid and glutamic acid. Less stimulation was obtained with lysine, glutamine, glycine or asparagine. Analyses of yeast phase cell wall amino acids showed H. capsulatum serotypes 1,2,3 differed from serotypes 1,4 by a higher molar concentration of lysine, arginine and threonine but lower glycine plus serine. Relative molar concentrations of the individual amino acids of H. capsulatum serotype 1,4 and H. duboisii were similar; the latter had a higher threonine content. The amino acids present in the mycelial cell walls of H. capsulatum serotype 1,2,3, serotype 1,4, and H. duboisii were not significantly different. No results completely distinguished between H. capsulatum serotype 1,4 and H. duboisii. A simple chemical test for identifying Histoplasma 1,4 serotypes is described.