Cultivation of Rat Molar Tooth Germs in Carrel Flasks. I

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Abstract
Forty sections of rat embryo mandibles or maxillas, containing tooth germs were cultivated. The explants ranged from 18 to 21 days embryonic age. In all cases the coagulum was chicken plasma with either rat or chick embryo extract. The explants degenerated in the central area, although survival was better with rat embryo extract. 103 dissected tooth germs were similarly studied. Degeneration occurred with the chick embryo extract. Using rat embryo extract, cultivation of dissected tooth germs 18 to 21 days embryonic age was successful up to 20 days in the flask. No transfers were made. Approx. 2 days development occurred in 6 days of cultivation. Further in vitro culture showed only growth and survival.