Indirect analysis of corticosteroids. 2. The determination and identification of urinary 17-hydroxy-20-oxosteroids unsubstituted at C(21)

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Abstract
A method was developed for the group determination of urinary 17-hydroxy-20-oxosteroids unsubstituted at C21 by their selective conversion into 17-oxosteroids and measurement of the latter as chromogens in the Zimmermann reaction. The conversion was brought about by successive treatment with sodium bismuthate, sodium borohydride, and sodium bismuthate. An extract of acid-hydrolyzed urine from a patient with adrenogenital syndrome was treated successively with bismuthate, borohydride and bismuthate: 3[alpha]-hydroxy-5-androstan,17-one was isolated from the mixture of products obtained. It was concluded that the isolated compound derives exclusively from native 3[alpha] : 17[alpha] -dihydroxypregnan-20-one.