A COMPARISON OF FLUORIMETRIC AND COLORIMETRIC METHODS FOR THE DETERMINATION OF α‐MANNOSIDASE ACTIVITY IN BOVINE PLASMA

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Abstract
Manual colorimetric and fluorometric methods for the assay of acidic .alpha.-mannosidase activity in bovine EDTA plasma are compared. The effect of variable blood volumes per 10 ml blood collecting tube was investigated and the colorimetric assay conditions adjusted (e.g., Zn concentration in pre-incubation buffer) to cope with low hemolyzed EDTA plasma samples; the 2 methods give similar results, but for hemolyzed samples, the colorimetric method yields significantly lower results than those obtained fluorometrically. Using the manual fluorometric method, samples can be assayed more rapidly and simply than by using the colorimetric method. This provides definite advantages in a laboratory receiving variable numbers of samples or which cannot afford the high cost of automated equipment. [Determination of .alpha.-mannosidase is important in the detection of heterozygotes for bovine mannosidosis.].