The preparation of [6,7-3H2]oestrogens

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Abstract
3-Acetoxyoestra-1,3,5(10),6-tetraen-17-one was catalytically reduced in an atmosphere of tritium-hydrogen gas to yield [6,7-3H2]oestrone acetate which, on hydrolysis, gave [6,7-3h2]-oestrone. In two experiments the radiochemical yields were 34.2 and 15.5% respectively. [6,7-3h2]-oestrone of high specific activity (20.2 mc/mg. or 5.45 c/m-mole) was obtained in the second (3c of tritium gas) run. [6,7-3H2]Oestradiol-17[beta] was prepared from [6,7-3H2]oestrone by reduction with sodium borohydride. [6,7-3H2]Oestriol-16[alpha],17[beta] and [6,7-3H2]oestriol-16[alpha],17[alpha] were prepared from [6,7-3H2]oestrone; the intermediate products of this reaction sequence, 3,17[beta]-diacetoxy[6,7-3H2]oestra-l,3,5(10),16-tetraene and 3,17[beta]-diacetoxy-16[alpha],17[alpha]-epoxy[6,7-3H2]-oestra-1,3,5(10)-triene, were isolated and characterized.