SaccharomycescerevisiaeMitoproteome Plasticity in Response to Recombinant Alternative Ubiquinol Oxidase
- 21 December 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 5 (2) , 339-348
- https://doi.org/10.1021/pr050346e
Abstract
The energy-dissipating alternative oxidase (AOX) from Hansenula anomala was expressed in Saccharomyces cerevisiae. The recombinant AOX was functional. A comparative analysis by two-dimensional differential in-gel electrophoresis (2D-DIGE) of mitochondrial protein patterns found in wild-type and recombinant AOX strains was performed. 60 proteins exhibiting a significant difference in their abundance were identified. Interestingly, proteins implicated in major metabolic pathways such as Krebs cycle and amino acid biosynthesis were up-regulated. Surprisingly, an up-regulation of the respiratory-chain complex III was associated with a down-regulation of the ATP synthase complex. Keywords: alternative oxidase • yeast • mitoproteome • proteomics • two-dimensional differential in-gel electrophoresisKeywords
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