Combination of metallic impregnation and autoradiography of brain sections
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 59 (2) , 111-116
- https://doi.org/10.1007/bf00518506
Abstract
After labelling with 14C-thymidine, frozen sections or paraffin sections of the brain of adult mice or rats were first stained by metallic impregnation and then coated with chrome alum gelatine and with an emulsion layer of about 10 μm. On the autoradiographs 14C-tracks are readily recognized above labelled astrocytes or oligodendrocytes, and these can be well discriminated, if the sections are processed by the silver carbonate method of Rio-Hortega. In contrast, no labelling is obtained, if the gold chloride sublimate method of Cajal is applied.This publication has 21 references indexed in Scilit:
- A TWO EMULSION AUTORADIOGRAPHIC TECHNIQUE AND THE DISCRIMINATION OF THE THREE DIFFERENT TYPES OF LABELLING AFTER DOUBLE LABELLING WITH 3H- AND WC-THYMIDINECell Proliferation, 1976
- Autoradiographic investigations of glial proliferation in the brain of adult mice. II. Cycle time and mode of proliferation of neuroglia and endothelial cellsJournal of Comparative Neurology, 1975
- Comparative study of astrocytes and mononuclear leukocytes reacting to brain trauma in miceExperimental Neurology, 1973
- Investigation of glial cells in semithin sections. I. Identification of glial cells in the brain of young ratsJournal of Comparative Neurology, 1973
- An evaluation of various double labelling and autoradiographic techniques for the measurement of DNA synthesis time in leukaemic cells*Journal of Microscopy, 1972
- DNA SYNTHESIS TIME IN LEUKAEMIC CELLS AS MEASURED BY THE DOUBLE LABELLING AND THE PERCENTAGE LABELLED MITOSES METHODSCell Proliferation, 1971
- Electron microscopic features and proliferation of astrocytes in the corpus callosum of the ratJournal of Comparative Neurology, 1969
- Identification of microglia in light and electron microscopyJournal of Comparative Neurology, 1969
- In-vitro Study of DNA-Synthesis Time and Cell-Cycle Time in Erythrocyte Precursors of Normal and Thalassaemic Subjects, Using a 3H- and 14C-Thymidine Double Labelling TechniqueActa Haematologica, 1967
- TWO-EMULSION RADIOAUTOGRAPHYJournal of Histochemistry & Cytochemistry, 1962