Effect of mouse phagocytes on Candida albicans in in vivo chambers

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Abstract
Mouse phagocytic cells in in vivo diffusion chambers had either candidacidal or candidastatic activity depending on the kind of phagocyte studied, the type of mouse whence the phagocytes came, and whether the chamber inoculum of Candida albicans consisted primarily of yeasts or hyphae. Killing of C. albicans occurred when yeasts were placed into chambers with membranes with 3.0-micrometer pores and implanted intraperitoneally into normal mice or thymus-deficient (nude) mice. Although C. albicans remains in chambers with 3.0-micrometer pores, host phagocytic cells can migrate into the chambers. Killing also occurred when yeasts were combined with normal or nude mouse neutrophils in chambers made with membranes with 0.45-micrometer pores, which restrict migration of host cells, but not diffusion of soluble factors. Populations of cells rich in macrophages were candidastatic for yeasts when the phagocytes came from normal mice but candidacidal when obtained from nude mice. Results of gradient fractionations of peritoneal exudates indicated that more than one cell type may be responsible for candidacidal activity by nude mouse macrophage-rich cells. Hyphal-phase cells of C. albicans appear to be more resistant than yeast-phase cells to killing by normal and nude mouse phagocytic cells.