Genetics of Ustilago violacea. XII. Half-Tetrad Analysis and Double Selection

Abstract
Mutations for fungicide resistance were characterized by the teliospore test as dominant, semidominant, or recessive. Chloroneb-resistance mutations are dominant, semidominant, or recessive; botran and carboxin-resistance mutations are dominant or semidominant; and thiabendazole-resistance mutations are semidominant. Chloroneb-resistance mutations occurred in at least 5 loci, botran-resistance mutations in at least 2 loci, and botran- and carboxin-resistance mutations in at least 1 locus. Centromere-linkage values were calculated for 37 mutations by half-tetrad analysis. Double selection to detect linkage indicated that U. violacea may have a relatively large number of linkage groups. The teliospore test, half-tetrad analysis and double selection can be used in the genetic study of other species of Ustilago.