Control of recA dependent activities in Escherichia coli: a possible role for the recF product

Publisher Website
Google Scholar
Abstract
DNA repair and genetic recombination were studied in recF143 mutants of E. coli in which expression of inducible, SOS repair activities was altered by additional mutations in lexA or recA. recF143 and lexA3 appeared to act additively to increase sensitivity to UV irradiation and to reduce recombination proficiency. The recF defect was suppressed in strains carrying the tif-1 allele of recA, but not in strains carrying mutations that increased synthesis of recA+ protein or which directly inactivated lexA repressor. These and other data are interpreted to suggest that the recF defect is related to a reduced activity of recA protein. The implications of these findings are discussed in relation to the control of SOS activities and cell division during normal growth.