Comparison of Enzyme-Linked Immunosorbent Assay Procedures for Detection of Tomato Ringspot Virus in Woody and Herbaceous Hosts

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Abstract
Two enzyme-linked immunosorbent assay (ELISA) procedures, direct and indirect, were compared in combination with two sample preparation methods, leaf disks and sap extraction to determine their relative abilities to detect tomato ringspot virus (TmRSV) in leaves of Chenopodium quinoa, geraniums, apples and peaches. In sap extracts, TmRSV was detected equally well by both ELISA procedures. When leaf disks were tested, the indirect procedure was somewhat more sensitive. Single leaf disks did not provide sufficient TmRSV for efficient detection by either ELISA method; however, increasing the number of disks to five facilitated efficient detection of TmRSV in geraniums and C. quinoa.