A Simplified Procedure for Obtaining Follicle-Stimulating Hormone from Sheep Pituitary Glands.

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Abstract
A short simple method is given for obtaining from sheep pituitary glands a follicle-stimulating hormone prepn. of high biological purity and low protein content. This method consists of extracting the glands with saturated NaCl soln. at 72 C, adjusting the extract to pH 2.5, dialysis of the soluble fraction, and recovery of the hormone from the dialyzed fraction by lyo-philization or by the use of an anion exchanger. When 5 g.eq. were given to male and female hypophysectomized rats for 10 days, only a very small amt. of luteinizing activity was found. The fraction insoluble at pH 2.5 contained both follicle-stimulating and luteinizing activities. Results are discussed from the viewpoint that follicle-stimulating hormone may exist in pituitary tissue in different forms.

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