PREPARATION AND PURIFICATION OF HUMAN INSULIN-I131; BINDING TO HUMAN INSULIN-BINDING ANTIBODIES

Open Access
Publisher Website
Google Scholar
Abstract
The preparation of I131-labeled human insulin from a lot of human insulin containing approximately 25% insulin by weight and its purification from labeled contaminants are described. The reaction of human insulin-I131 with insulin-binding antibodies in the serums of human subjects treated with commercial mixtures of animal insulins is demonstrated directly. Comparison of the binding of human insulin and beef insulin in antiserums from 8 insulin resistant and non-resistant diabetic subjects revealed a lesser affinity of antibody for human than for beef insulin in most cases but considerable variability in this resepct was encountered among different antiserums. It is suggested that the ability of certain diabetic patients (who show evidence of insulin resistance to exogenous insulin) to tolerate withdrawal of insulin therapy, with or without the aid of sulfonylurea agents, is related to the relatively weak binding to antibody of endogenously secreted human insulin.