ULTRAVIOLET LIGHT-INDUCED TRANSFORMATION OF HUMAN-CELLS TO ANCHORAGE-INDEPENDENT GROWTH

1 January 1980

journal article
research article

Vol. 40 (6) , 1934-1939

Abstract

A system for UV light transformation of human embryonic cells to anchorage-independent growth is presented. The procedure involves multiple UV irradiations, post-irradiation growth and plating in soft agar. Transformants are obtained at frequencies from 1-80/105 cells at UV exposures to 25 J/m2. The resulting transformants can be subcultured on solid surfaces. The cells show crisscrossing and piling up; they reach 2- to 5-fold higher saturation densities than the parental cells. Some subcultures show increased plating efficiency in soft agar and increased life span. The susceptibility of the UV transformation process to apparent photoenzymatic reversal implies that pyrimidine dimers play a role in its induction.

This publication has 9 references indexed in Scilit:

Neoplastic transformation of human diploid fibroblast cells by chemical carcinogens
Proceedings of the National Academy of Sciences, 1978
CARCINOGENESIS IN TISSUE-CULTURE .29. NEOPLASTIC TRANSFORMATION OF ABNORMAL HUMAN DIPLOID CELL STRAIN, WI-38, WITH CO-60 GAMMA-RAYS
1978
Evidence that pyrimidine dimers in DNA can give rise to tumors.
Proceedings of the National Academy of Sciences, 1977
Photoreactivation of thymine dimers in UV-irradiated human cells: Unique dependence on culture conditions
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1977
Culture conditions affect photoreactivating enzyme levels in human fibroblasts
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
Xeroderma Pigmentosum
Annals of Internal Medicine, 1974
The chemical nature of photoreactivable lesions in DNA.
Proceedings of the National Academy of Sciences, 1965
A Small and Inexpensive Ultraviolet Dose-Rate Meter Useful in Biological Experiments
Radiation Research, 1961
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Journal of Biological Chemistry, 1951