Analysis of the structure and variability of nucleolar organizer regions of Salmo trutta by C-, Ag-, and restriction endonuclease banding

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Abstract
Nucleolar organizer regions (NORs) of brown trout were investigated using C-, Ag-, and restriction endonuclease banding. The presence of constitutive heterochromatin was confirmed by C-banding. Giemsa-staining, C-banding, and Ag-banding revealed great variability in the size of the short arm of the NOR-bearing chromosome. This size variation was due in some cases to NOR duplication. Restriction endonuclease digestion induced a specific banding pattern for Alul, Ddel, Haelll, Mbol, and Hinñ, indicating some features about the sequence composition of the NOR-associated heterochromatin.