EFFECTS OF GIBBERELLIC ACID ON BARLEY ENDOSPERM
Open Access
- 8 July 1962
- journal article
- research article
- Published by The Institute of Brewing & Distilling in Journal of the Institute of Brewing
- Vol. 68 (4) , 322-332
- https://doi.org/10.1002/j.2050-0416.1962.tb01873.x
Abstract
When slices of barley endosperm are incubated with gibberellic acid in aseptic conditions the activities of salt-soluble endo-β-glucanase and endopentosanase are greatly augmented and enzymes and soluble products of cell-wall degradation (hexosan and pentosan) are secreted into the surrounding medium. This cytolytic action, together with the enhanced amylolytic activity which can be detected in isolated endosperms subjected to gibberellic acid, is sufficient to cause complete solubilization of the starchy endosperm in 3 days at 25° C. Gibberellic acid acts through the respiring aleurone layer; it has no effect on aleurone-free endosperm or on endosperm slices in which the aleurone has lost the power of reducing tetrazolium salts. Gibberellic acid-induced sugar secretion from endosperm slices is maximal at 30° C. and completely inhibited at 37° C. and secretion both of sugars and of endo-β-glucanase is unaffected in conditions which inhibit proteolysis. Excised barley embryos show no response to added gibberellic acid. It is suggested that, when barley germinates without additions of gibberellic acid, endogenous gibberellin present in the embryo is translocated to the aleurone where it induces secretion of hydrolytic enzymes from subcellular particles, so causing modification to proceed inwards from the aleurone; if this is so, then added gibberellic acid merely enhances the effects of the endogenous component.Keywords
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