Simplified Spectrophotometric Assay of Serum Glutamic Oxaloacetic Transaminase and Lactic Dehydrogenase

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Abstract
This paper deals with an economical, simplified modification of the methods for determining the levels of glutamic oxaloacetic transaminase (S-GOT) and lactic dehydrogenase (S-LD) in plasma (or in serum). A single blank of reduced cozymase (DPN-H) may be used for numerous determinations on test specimens. The modified procedure yields reliable and accurate results, and it is saving of time and reagents. By means of defining a unit of S-LD as the amount that produces a change of 0.01 in the optical density, data for S-LD may be adjusted so that they are numerically comparable with those for S-GOT. Comparison of levels of S-GOT and S-LD in 10 patients with acute infarcts of the myocardium indicated that the activity of both enzymes was greater than normal. Although the patterns of activity of the 2 enzymes were closely correlated, the level of S-LD reached a peak later, and returned to normal later than that for S-GOT.

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