Phosphoinositides in Barley Aleurone Layers and Gibberellic Acid-Induced Changes in Metabolism

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Abstract
Phospholipids of barley (Hordeum vulgare L. cv. Himalaya) aleurone layers were labeled with myo-[2-3H]inositol or [32Pi], extracted, and analyzed by physical (chromatography) and chemical (deacylation) techniques. Three phospholipids were found to incorporate both myo-[2-3H]inositol and [32Pi]-phosphatidylinositol, phosphatidylinositol-monophosphate, and phosphatidylinositol-bisphosphate. Stimulation of [3H]inositol prelabeled aleurone layers with GA3 showed enhanced incorporation of label into phosphatidylinositol within 30 seconds and subsequent rapid breakdown. Stimlation of phosphatidylinositol labeling observed in these studies is the earliest response of aleurone cells to gibberellic acid reported.