Cloning and characterization of the gene encoding 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli
Open Access
- 1 October 1986
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 168 (1) , 437-439
- https://doi.org/10.1128/jb.168.1.437-439.1986
Abstract
The cloning of the gene for Escherichia coli PL-2 2-keto-3-deoxy-D-manno-octonate 8-phosphate synthetase is reported. Positive transformants showed an increase of approximately three-fold in specific activity of the enzyme both in E. coli and in Salmonella typhimurium as host cells. A subclone containing a 1.5-kilobase PvuII fragment overproduced active enzyme. Minicell experiments that allow the detection of plasmid encoded proteins revealed an insert-coded single protein band of 34 kilodaltons.This publication has 17 references indexed in Scilit:
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