Abstract
Vibrio strains isolated from plankton, sediments, and seawater in two coastal areas south of Valenica, Spain, were genotypically and phenotypically different from other Vibrio species. The guanine-plus-cytosyine content of the strains was 43 mol%. Deoxyribonucleic acid-deoxyribonucleic acid hybridization studies revealed that the strains were genetically homogeneous and exhibited very low levels of genetic relatedness to Vibrio parahaemolyticus ATCC 17802T (T = type strain) (0%), Vibrio harveyi NCMB 1280T (11%), Vibrio vulnificus NCMB 2046T (7%), Vibrio nereis NCMB 1897T (0%), Vibrio natriengens NCMB (9%), and Vibrio splendidus NCMB 1T (0%). Strain CECT 621 of the Coleccion Espanola de Cultivos Tipo was designated the type strain of the proposed new species Vibrio mediterranei.