Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli
- 1 March 1985
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 161 (3) , 1219-21
- https://doi.org/10.1128/jb.161.3.1219-1221.1985
Abstract
A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with genes of either chromosomal or plasmid origin.Keywords
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