Differential regulation of an auxin-producing nitrilase gene family in Arabidopsis thaliana.
- 5 July 1994
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (14) , 6649-6653
- https://doi.org/10.1073/pnas.91.14.6649
Abstract
Nitrilases (nitrile aminohydrolase, EC 3.5.5.1) convert nitriles to carboxylic acids. We report the cloning, characterization, and expression patterns of four Arabidopsis thaliana nitrilase genes (NIT1-4), one of which was previously described [Bartling, D., Seedorf, M., Mithöfer, A. & Weiler, E. W. (1992) Eur. J. Biochem. 205, 417-424]. The nitrilase genes encode very similar proteins that hydrolyze indole-3-acetonitrile to the phytohormone indole-3-acetic acid in vitro, and three of the four genes are tandemly arranged on chromosome III. Northern analysis using gene-specific probes and analysis of transgenic plants containing promoter-reporter gene fusions indicate that the four genes are differentially regulated. NIT2 expression is specifically induced around lesions caused by bacterial pathogen infiltration. The sites of nitrilase expression may represent sites of auxin biosynthesis in A. thaliana.Keywords
This publication has 21 references indexed in Scilit:
- Nitrilase in biosynthesis of the plant hormone indole-3-acetic acid from indole-3-acetonitrile: cloning of the Alcaligenes gene and site-directed mutagenesis of cysteine residues.Proceedings of the National Academy of Sciences, 1993
- Nitrilase from Rhodococcus rhodochrous J1. Sequencing and overexpression of the gene and identification of an essential cysteine residue.Journal of Biological Chemistry, 1992
- Primary structure of an aliphatic nitrile-degrading enzyme, aliphatic nitrilase, from Rhodococcus rhodochrous K22 and expression of its gene and identification of its active site residueBiochemistry, 1992
- Cloning and expression of an Arabidopsis nitrilase which can convert indole‐3‐acetonitrile to the plant hormone, indole‐3‐acetic acidEuropean Journal of Biochemistry, 1992
- Indole-3-Acetic Acid Biosynthesis in the Mutant Maize orange pericarp , a Tryptophan AuxotrophScience, 1991
- Requirement of the Auxin Polar Transport System in Early Stages of Arabidopsis Floral Bud Formation.Plant Cell, 1991
- Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.Proceedings of the National Academy of Sciences, 1991
- Fast and sensitive multiple sequence alignments on a microcomputerBioinformatics, 1989
- Genomic sequencing.Proceedings of the National Academy of Sciences, 1984
- Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-regionPlasmid, 1982