Kinetics of heat inactivation of Venezuelan Equine Encephalomyelitis Virus

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Abstract
Thermal inactivation of Venezuelan Equine Encephalomyelitis Virus (VEEV) was studied at temperatures from 26° to 55° C. Inactivation of infectivity took place by two thermodynamically different reactions, one of which predominated at temperatures below 44° C and the other at higher temperatures. The presence of 1 or 2m NaCl stabilized the VEE virus at low temperatures but enhanced the inactivation at high temperatures. This latter effect at temperatures higher than 50° C, is associated with the occurrence of two-component survival curves. The different effects of hypertonic NaCl concentrations at the two ranges of temperature, are related to different mechanisms of inactivation operating at each range (protein denaturation and nucleic acid-RNA breakdown). Different kinetics of thermal inactivation at 55° C were observed between virus strains with different virulence. However, no significant correlation was found between the virulence of the eleven VEE virus strains studied and their thermostability at 37° and 55° C.

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