Chromatin structure in the cellular slime mold Dictyostelium discoideum.
- 1 February 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (2) , 705-709
- https://doi.org/10.1073/pnas.75.2.705
Abstract
The structure of Dictyostelium discoideum chromatin has been studied by the following techniques: electron microscopy, staphylococcal nuclease digestion, acrylamide gel electrophoresis, sucrose gradient centrifugation, and melting. The basic unit of chromatin is the nucleosome, which is a particle 98.6 A in diameter. Approximately 50% of the chromatin is protected from nuclease digestion, but this decreases when protease activity is not inhibited. The nucleosome contains 187 base pairs of DNA, including a 137-base-pair core and a 50-base-pair linker. The monomer nucleosome has an s20,w value of 11.5 S on isokinetic sucrose gradients. When the chromatin is melted, four transitions are observed, at 54.5 degrees, 66.7 degress, 74.9 degrees, and 79.7 degrees. The structure of Dictyostelium chromatin is very similar to that seen in higher eukaryotes.This publication has 34 references indexed in Scilit:
- Structure of nucleosome core particles of chromatinNature, 1977
- Physical properties of chemically acetylated rat liver chromatin.Proceedings of the National Academy of Sciences, 1977
- Structure of ChromatinAnnual Review of Biochemistry, 1977
- A defined minimal medium for axenic strains of Dictyostelium discoideumProceedings of the National Academy of Sciences, 1977
- Involvement of histone H1 in the organization of the chromosome fiber.Proceedings of the National Academy of Sciences, 1977
- Chromatin structure in the nuclei of the ciliate Stylonychia mytilusBiochemical and Biophysical Research Communications, 1977
- A comparison of the structure of chicken erythrocytef and chicken liver chromatinCell, 1976
- Organization of the ribosomal RNA genes of dictyostelium discoideum: Mapping of the nontrascribed spacer regionsCell, 1976
- The DNA repeat lengths in chromatins from sea urchin sperm and gastrula cells are markedly differentFEBS Letters, 1976
- Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic AcidNature, 1953