Effect of 2-Deoxy-D-Glucose on Plasma Somatostatin Levels in Conscious Dogs*

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Abstract
The effects of 2-deoxy-D-glucose (2-DG) on plasma levels of somatostatin-like immunoreactivity (SLI) were examined in conscious normal dogs. After an iv infusion of 2-DG(400 mg/kg·h for 15 min), plasma SLI rose significantly from a mean baseline of 130 ± 5 pg/ml (mean ± SEM) to a mean peak of 204 ± 25 pg/ml (P < 0.005) at 25 min. Plasma insulin and glucagon also increased significantly. Atropine (200 μg/kg·h for 35 min, iv) and hexamethonium (5 mg/kg, iv) markedly suppressed the SLI response to 2-DG, suggesting that it might be mediated, at least in part, by the autonomic nervous system. In contrast, the plasma insulin and plasma glucagon responses to this glucose analog were only slightly affected by atropine or hexamethonium pretreatment. Carbachol (0.2 mg, sc) caused a mean maximal increase in SLI of 43 ± 14% (P < 0.005) and atropine (200 μg/kg·h, iv) caused a mean maximal decrease of 25 ± 2% (P < 0.001) from the resperesctive baseline levels. Plasma insulin and glucagon rose promptly after carbachol and were unchanged by atropine. To assess the contribution of 2-DG-stimulated gastric acid secretion in the 2-DG-induced SLI rise, 2-DG was infused during the infusion of the H2-receptor antagonist cimetidine (3.0 mg/kg·h). Plasma SLI, nevertheless, increased significantly from a mean baseline of 112 ± 6 pg/ml to a mean peak of 158 ± 10 pg/ml (P < 0.005) at 20 min, although the magnitude of the response was substantially reduced (P =NS). These observations suggest that in the conscious dog, 2-DG stimulates SLI secretion in part via cholinergic mechanisms.