The Na+/e− stoichiometry of the Na+‐motive NADH : quinone oxidoreductase in Vibrio alginolyticus
Open Access
- 16 June 1997
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 409 (3) , 475-477
- https://doi.org/10.1016/s0014-5793(97)00536-x
Abstract
A method is proposed to estimate the stoichiometries of primary Na+-pumps in intact bacterial cells. It is based on technique when the H+/e− stoichiometry is measured in the presence of protophorous uncoupler and in the absence of penetrating ions other than H+. Under these conditions, the H+ influx discharges membrane potential generated by the Na+ pump so the Na+/e− and H+/e− ratios become equal. Using this approach it is shown that the Na+/e− ratio for the Na+-motive NADH : quinone oxidoreductase of Vibrio alginolyticus is equal to 0.71±0.06. The Na+/e− stoichiometry appears to be ≈1, provided that the contribution of the non-coupled NADH : quinone oxidoreductase, which is resistant to low HQNO concentrations, is taken into account.Keywords
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