Immunoperoxidase detection of T and B cells in blood compared with conventional methods.

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Abstract
Peripheral blood T and B cells were enumerated in 26 normal adults by conventional immunological markers (erythrocyte rosette and surface membrane Ig) and by monoclonal markers (T11 and B1) using both membrane immunofluorescence of cells in suspension and immunoperoxidase staining of dried, fixed, cytocentrifuged cells afer separation from blood by buoyant density centrifugaton. The results of immunochemistry were determined independently from the results of erythrocyte rosetting and membrane immunofluorescence techniques. A high correlation was found between all 3 methods of enumerating T cells (erythrocyte rosetting, T11/immunoperoxidase and T11/indirect immunofluorescence). Correlation was lessened between the results of the 3 methods for enumerating B cells; surface membrane Ig and B1/indirect immunofluorescence techniques showed the highest correlation. B cells may be preferentially lost during the extensive cell washings entailed in the indirect immunofluorescence procedures. Immunochemical methods, using monoclonal antibodies, are useful for enumerating lymphocyte subsets in blood, particularly when permanent records are desired.