Neuronal‐glial interactions: Complexity of neurite outgrowth correlates with substrated adhesivity of serotonergic neurons

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Abstract
To study the interactions between neurons of known transmitter phenotype and non-neuronal cells of glial or fibroblastic origin, serotonergic (5-HT) neurons were tested for their strength of adhesion and neurite outgrowth patterns on substrates of astrocytes or fibroblasts using a cell adhesion assay for transmitter-identified neurons, and morphometry of immunocytochemically stained neurons in dissociated cell cultures. Both the strength of adhesion and the rate and complexity of neurite outgrowth by 5-HT neurons were significantly greater on substrates of astrocytes compared to fibroblasts. These results provide evidence that 5-HT neurons can interact selectively with glia via cell surface determinants, and that this process may be important for the development of complex (dendrite-like) neuritic arbors. The methods developed in this study will be useful for future studies of interactions between transmitter-identified neurons and glial cells during ontogeny of the embryonic brain.