A New Method for Detecting Penicillinase Production by Staphylococci

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Abstract
A blood-agar plate is uniformly streated with a penicillin-sensitive strain of Staphylococcus aureus. Filter paper disks containing penicillin are placed at intervals on the plate. All but one of the disks are treated with cultures of strains suspected of having penicillinase activity. Penicillinase production results in a lack of rings of inhibition.