Chondroitin sulphate covalently cross‐links the three polypeptide chains of inter‐α‐trypsin inhibitor
- 1 April 1994
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 221 (2) , 881-888
- https://doi.org/10.1111/j.1432-1033.1994.tb18803.x
Abstract
Inter-alpha-trypsin inhibitor (ITI) is a tight complex of three different proteins: bikunin and two heavy chains H1 and H2. In order to demonstrate that the three chains are covalently linked by a chondroitin sulphate chain as previously proposed [Enghild, J. J., Salvesen, G., Hefta, S. A., Thogersen, I. B., Rutherford, S. and Pizzo, S. V. (1991) J. Biol. Chem. 266, 747-751], ITI was extensively digested with thermolysin and the glycosaminoglycan-containing fragment was isolated from the digest by ion-exchange chromatography. Its peptide structural determination and mass spectrometry analysis both provide evidence that the different peptide chains constituting ITI are associated by the new cross-link described as the protein-glycosaminoglycan-protein cross-link.Keywords
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