A Simple Method to Demonstrate Lipids in Epon-Embedded Ultrathin Sections
- 1 January 1970
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 45 (3) , 129-132
- https://doi.org/10.3109/10520297009085354
Abstract
Growing oocytes of the marine crab Cancer pagurus L. contain different kinds of yolk inclusions. Some of them are strongly blackened by OsO4 and presumably are lipids. The lipid character of these granules was demonstrated by extracting them from the epon-embedded ultrathin sections by using the method of Mayor et al. (J. Biophys. Biochem. Cytol., 9:909, 1961) originally recommended for the removal of epoxy resin from sections intended for light microscopy. This technique was also applied to liver tissue of mouse with successful result.This publication has 6 references indexed in Scilit:
- THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPYThe Journal of cell biology, 1963
- THE ROLE OF FIXATION IN ELECTRON STAININGJournal of the Royal Microscopical Society, 1963
- A SIMPLE METHOD FOR REMOVING THE RESIN FROM EPOXY-EMBEDDED TISSUEThe Journal of cell biology, 1961
- IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODSThe Journal of cell biology, 1961
- Staining of Tissue Sections for Electron Microscopy with Heavy MetalsThe Journal of cell biology, 1958
- A STUDY OF FIXATION FOR ELECTRON MICROSCOPYThe Journal of Experimental Medicine, 1952