Non‐parallel enzyme secretion from rat pancreas in vitro studies.

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Abstract
The relative variations of rat pancreatic amylase vs. lipase and chymotrypsinogen secretions were studied in vitro with the help of 2 different techniques: in situ organ perfusion and incubation of pancreatic lobules. In experiments on the perfused pancreas, with 8 C u [unit] kg-1/hr secretin added to the perfusion fluid, cholecystokinin-pancreozymin (8 ID [intraduodena] u/kg) or pilocarpine (15 mg kg-1) both resulted in a significant change in the enzyme proportions in the juice. In experiments on pancreatic lobules, the addition to the incubation medium of secretin (10-7 M), alone or associated with cholecystokinin-pancreozymin (8 .times. 10-7 M) or pilocarpine (10-4 M) did not induce any change in the enzyme proportions in secretion. The non-parallelism between enzyme secretions can occur in the rat upon pancreozyminic or cholinergic stimulation in vitro as well as in vivo provided basal protein output is low enough. This is not the case when the tissular integrity of the pancreas is lost, such as in experiments on lobules. Pancreatic secretion derives from 2 intrapancreatic pools of different enzymatic composition. A possible explanation for some discrepancies on the existence of non-parallel secretion is given.