HLA Antigens in a Psoriatic Family: Comparative Studies with MLC, HTC, PLT and Serological HLA-DR Determinations

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Abstract
HLA genotypes were characterized in a large family of 48 individuals in 3 generations. Carriers of the proband''s disease-predisposing haplotype commonly expressed clinical signs of illness, manifested as psoriasis and/or arthritic lesions. These data and other family studies led to the conclusion that cutaneous and/or joint lesions may be signs of disease in carriers of the predisposing HLA haplotype. HLA-A, B, C and D/DR antigens were investigated in the family members to evaluate the validity of different assays for the determination of HLA-D alleles in a family where MLC [mixed lymphocyte culture] tests were the basis for a correct assignment of HLA-D determinants. The HTC [homozygous typing cells] method and the PLT (primed lymphocyte typing) assay were both afflicted with specific typing problems, partly due to the existence of cellular cross-reactions between HLA-D determinants somewhat like serological DR cross-reactions.