Simultaneous spectrofluorimetric determination of glycerol and ethanol in wine by flow injection using immobilized enzymes

Publisher Website
Google Scholar
Abstract
An automatic spectrofluorimetric method for the simultaneous determination of glycerol and ethanol is presented. The method is based on the use of a flow injection (FI) manifold and dehydrogenase enzyme immobilized on controlled-pore glass. The proposed method allows for the simultaneous determination of glycerol (linear range between 1 and 10 µg ml–1, r= 0.9989 and sr –1, r= 0.9998 and sr –1. The results obtained on eight wines were compared with those provided by conventional methods and the agreement between them corroborated the usefulness of the proposed method.