PURIFICATION OF AN ALKALINE PHOSPHATASE-LIPOPROTEIN-X COMPLEX

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (5) , 604-608
Abstract
An alkaline phosphatase isoenzyme was observed in an abnormal position in the agar-agarose gel pattern of sera from several patients suffering from intrahepatic and extrahepatic cholestasis. This isoenzyme was purified, by gel filtration and affinity chromatography, from the serum of a patient suffering from extrahepatic cholestasis. Analysis demonstrated an alkaline phosphatase-lipoprotein-X complex with a relative molecular mass of at least 669,000. The interpretation of alkaline phosphatase isoenzyme patterns produced by different techniques was discussed.