Evaluation of Two Simple Assay Methods for Detecting Antibiotic Residues in Chicken and Pig Muscle
Open Access
- 1 June 1975
- journal article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 38 (3) , 235-243
- https://doi.org/10.1111/j.1365-2672.1975.tb00528.x
Abstract
Using 2 paper disc assay procedures, Bacillus cereus and Sarcina lutea were challenged with extracts from chicken and pig muscle paste to which various antibiotics had been added. The first method could detect tetracycline, chlortetracycline, oxytetracycline, penicillin, erythromycin, tylosin and virginiamycin at or <1 μg/g of muscle paste, whilst the second method was necessary to detect the remaining 5 antibiotics at this level. Using the first assay procedure, penicillin, zinc bacitracin and the 3 tetracycline compounds could be detected below FAO/WHO maximum permissible levels for meat for human consumption, whilst the second method was necessary to meet requirements for streptomycin, erythromycin and tylosin. Spiramycin could not be detected by either method at the levels permitted by FAO/WHO. The minimum levels of detection obtained for flavomycin, virginiamycin and monensin were 0.2, 0.02 and 0.5–1.0 μg/g, respectively.Keywords
This publication has 3 references indexed in Scilit:
- Antibiotic Feeding of Farm Animals and Resistance Factors in BacteriaCanadian Institute of Food Science and Technology Journal, 1973
- Third Stenhouse‐Williams Memorial Lecture: Some Environmental Consequences of the Use of Antibiotics: Or ‘What goes up must come down’Journal of Applied Bacteriology, 1972
- Detection of Residual Antibiotics in Food (I)Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 1971