Evidence for a photoinduced synthesis of poly(A) containing mRNA in Fusarium aquaeductuum

1 January 1977

journal article
research article
Published by Springer Nature in Planta

Vol. 136 (1) , 45-48
https://doi.org/10.1007/bf00387923

Abstract

Based on inhibitor studies it has been concluded that in photoregulated processes of plant development, light induces differential gene expression. Using affinity chromatography of double labelled polysomal RNA on poly(U)-sepharose 4B, we were able to demonstrate different ³H/¹⁴C ratios for the bound poly(A) containing fraction (mRNA) when compared with the unbound fraction: when [³H]uridine was present in the light induced sample and [¹⁴C]uridine in the dark control, in the bound material the ³H/¹⁴C ratio was found to be higher than in the unbound fraction and vice versa. No such shift was observed, when both sample and control were kept in the dark. Our data are interpreted to provide evidence for photoinduced de novo synthesis of mRNA.

Keywords

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