Effects of fatty acids on carbohydrate and fatty acid metabolism of rat diaphragm

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Abstract
The long chain fatty acids palmitic acid and oleic acid, over a wide range of FFA [Free fatty acid] concentrations (<10-2,000 [mu]eq/liter) and FFA-to-albumin molar ratios ([image] 0.1-6.0), did not affect glucose uptake, 2-deoxyglucose transport and phosphorylation, glucose-U-Cl4 conversion to glycogen, [alpha]-amino-isobutyric acid transport, or the stimulatory effects of insulin on these processes in the isolated rat diaphragm obtained from normal animals. The tissue FFA level was not significantly affected by varying the extracellular palmitate level from 0 to 2,000 e[mu]q/liter (palmitate-albumin [image] 0.6-4.6). Furthermore, the kinetics of C14 palmitate entry into the tissue FFA pool in contrast to those of incorporation in the phospholipid and triglyceride fractions indicated that the tissue FFA pool is functionally heterogeneous and not rapidly responsive to changes in the extracellular FFA level.