Binding of the complement intermediate C56 to zymosan in acute phase human sera.
- 1 April 1975
- journal article
- Vol. 20 (1) , 113-24
Abstract
C56 is known to appear in the fluid phase when zymosan is incubated at 37 degrees C with certain acute phase 'reactor' sera. In the present study, C56 was detected bound to the zymosan particle prior to its appearance free in solution. In reactor sera C56 was formed and released with kinetics similar to that of the generation and decay of a C56-binding site formed when zymosan was incubated with normal serum. Bound and fluid phase C56 was detected only in reactor sera, and was generated only by agents known preferentially to activate the properdin pathway. Elution of C56 from zymosan in hypertonic salt solutions proved to be a convenient step in the partial purification of large amounts of this haemolytically active bimolecular complex.This publication has 15 references indexed in Scilit:
- Studies on the inhibition of C56-initiated lysis (reactive lysis). III. Characterization of the inhibitory activity C567-INH and its mode of action.1975
- Studies of Complement Complex C5b,6 Eluted from—EAC-6: Reaction of C5b,6 with EAC4b,3b and Evidence on the Role of C2a and C3b in the Activation of C5The Journal of Immunology, 1974
- Kinetic Studies of the Formation of the Properdin System Enzymes on Zymosan: Evidence That Nascent C3b Controls the Rate of AssemblyThe Journal of Immunology, 1974
- Studies on the Inhibition of C56-Initiated Lysis (Reactive Lysis). II. C567-INH – An Inhibitor of the C567 Trimolecular Complex of ComplementInternational Archives of Allergy and Immunology, 1974
- The reaction of zymosan with the properdin system in normal and C4-deficienct guinea pig serum. Demonstration of C3- and C5-cleaving multi-unit enzymes, both containing factor B, and acceleration of their formation by the classical complement pathway.1973
- Reaction mechanisms of nascent C567 (reactive lysis). I. Reaction characteristics for production of EC567 and lysis by C8 and C9.1972
- MOLECULAR ANALYSIS OF THE MEMBRANE ATTACK MECHANISM OF COMPLEMENTThe Journal of Experimental Medicine, 1972
- The fifth component of the guinea pig complement system. 3. Dissociation and transfer of C5b, and the probable site of C5b fixation.1971
- LYSIS OF ERYTHROCYTES BY COMPLEMENT IN THE ABSENCE OF ANTIBODYThe Journal of Experimental Medicine, 1970
- REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLSThe Journal of Experimental Medicine, 1970