Uncoupling reagents and metabolism. 5. Effects of salicylate and of 2,4-dinitrophenol on the metabolism of isolated rat diaphragm

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Abstract
The effects of 5 m[image]-salicylate and 0.25 m[image]-2,4-dinitrophenol on the incorporation of C14 from [Cl4]glucose, [2-Cl4]acetate and [3-C14]-pyruvate into the soluble intermediates of isolated rat-diaphragm muscle was studied. In the absence of the uncoupling reagents, the tissue preparations incorporated Cl4 into substances known to be involved in glycolysis, in transamination, in the tricarboxylic acid cycle and in synthetic reactions. When Krebs-Ringer phosphate was used as the incubation medium a greater proportion of radioactive intermediates was found in the separated incubation medium than when Krebs-Ringer bicarbonate was employed. The effects of salicylate and of 2,4-dinitrophenol on the distribution of C14 among the soluble intermediates were explicable in terms of their known actions on oxidative phosphorylation and on transamination. Salicylate and 2,4-dinitrophenol caused an increased loss of the labelled soluble intermediates from the tissue into the incubation media.

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