Characterization of Interferon Messenger RNA from Human Lymphoblastoid Cells
Open Access
- 1 July 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 44 (1) , 231-234
- https://doi.org/10.1099/0022-1317-44-1-231
Abstract
After treatment with Sendai virus, Namalwa cells form large amounts of interferon. RNA extracted from treated whole cells or from their polysomes was injected into Xenopus laevis oocytes and the interferon formed was titrated. The results show that the amount of interferon mRNA was maximal by 9 h after treatment of the cells with Sendai virus and then declined. Sucrose gradient centrifugation of the mRNA gave substantial purification and showed that its size was 12 S.This publication has 4 references indexed in Scilit:
- Production of human lymphoblastoid interferon by Namalva cellsJournal of Clinical Microbiology, 1978
- Induction and decay of human fibroblast interferon mRNA.Proceedings of the National Academy of Sciences, 1977
- Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells.Proceedings of the National Academy of Sciences, 1977
- Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liverBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966