Sensitivity and specificity of anti‐Jo‐1 antibodies in autoimmune diseases with myositis

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Abstract
Objective. To determine the sensitivity and specificity of anti-Jo-1 in systemic sclerosis (SSc) patients with and without myositis. Methods. Immunoblots on HeLa nuclei were used to screen sera from 554 consecutive connective tissue disease patients. Those who had 45–55-kd bands, all patients with polymyositis/dermatomyositis (PM/DM), and a random selection of SSc, Raynaud's disease, systemic lupus erythematosus, and rheumatoid arthritis patients were also studied by anti–Jo-1 enzyme-linked immunosorbent assay and by immunoblots on rabbit pooled aminoacyl-transfer RNA synthetase. Results. Anti–Jo-1 was present only in 8 of the 40 PM/DM patients. Conclusion. Anti–Jo-1 is specific for PM/DM.