The high affinity binding site on polyoma virus DNA for the viral large-T protein
- 1 January 1981
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (21) , 5697-5710
- https://doi.org/10.1093/nar/9.21.5697
Abstract
In order to map the high affinity binding site for the viral large-T protein on polyoma virus DNA, we have developed an assay which does not require purified protein. It is based on the specific elution of the large-T ATPase activity from calf thymus DNA cellulose by recombinant DNA molecules including known sequences of the viral DNA. Using this assay, a high affinity binding site has been mapped on the early region side of the ori region. Binding requires the integrity of a sequence /AGAGGC/TTCC/AGAGGC/ (nucleotides 49 to 64 in the DNA sequence of the A2 strain). Similar repeats of a PuGPuGGC sequence within less than 20 bases are not found within the viral coding regions, but are strikingly common in the control regions of papovaviruses and other eukaryotic DNAs.Keywords
This publication has 21 references indexed in Scilit:
- In vivo sequence requirements of the SV40 early promoter regionNature, 1981
- Regulatory mutants of polyoma virus defective in DNA replication and the synthesis of early proteinsCell, 1980
- Coding potential and regulatory signals of the polyoma virus genomeNature, 1980
- The genome of human papovavirus BKVCell, 1979
- The nucleotide sequence and genome organization of the polyoma early region: Extensive nucleotide and amino acid homology with SV40Cell, 1979
- Protein-DNA Interactions at the Origin of Simian Virus 40 DNA ReplicationPublished by Cold Spring Harbor Laboratory ,1979
- The binding site on SV40 DNA for a T antigen-related proteinCell, 1978
- (Na+, K+)-Activated Adenosinetriphosphatase of Axonal Membranes, Cooperativity and Control. Steady-State AnalysisEuropean Journal of Biochemistry, 1976
- Location of the Origin and Terminus of Replication in Polyoma Virus DNAJournal of General Virology, 1974
- The Replication of Polyoma DNAJournal of General Virology, 1973