A ubiquitous protein is the source of naturally occurring peptides that are recognized by a CD8+ T-cell clone.
- 1 December 1993
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (23) , 11272-11276
- https://doi.org/10.1073/pnas.90.23.11272
Abstract
We previously isolated from mouse spleen an octapeptide (LSPFPFDL) that in association with the class I major histocompatibility complex protein Ld is recognized by the antigen-specific receptor of an alloreactive CD8+ T-cell clone (2C). Guided by an assay dependent upon the same 2C T-cell receptor, we have now isolated from the same source another naturally occurring peptide. The second peptide (VAITRIEQLSPFPFDL) includes the entire octapeptide sequence and preliminary evidence suggests that it may be a natural precursor of the octapeptide. On finding extensive sequence homology between the 16-mer and part of human 2-oxoglutarate dehydrogenase, we determined the cDNA sequence of mouse 2-oxoglutarate dehydrogenase and found that the deduced amino acid sequence matches precisely the two naturally occurring peptides, indicating their origin by cellular processing of this ubiquitous self protein.Keywords
This publication has 27 references indexed in Scilit:
- Proteasome subunits encoded by the major histocompatibility complex are not essential for antigen presentationNature, 1992
- Peptide-dependent recognition of H–2Kb by alloreactive cytotoxic T lymphocytesNature, 1989
- Association of class I major histocompatibility heavy and light chains induced by viral peptidesNature, 1989
- Structure and regulation of KGD1, the structural gene for yeast alpha-ketoglutarate dehydrogenase.Molecular and Cellular Biology, 1989
- Antigen Recognition by Class I-Restricted T LymphocytesAnnual Review of Immunology, 1989
- A pentapeptide as minimal antigenic determinant for MHC class I-restricted T lymphocytesNature, 1989
- Studies of two antigenic forms of Ld with disparate beta 2-microglobulin (beta 2m) associations suggest that beta 2m facilitate the folding of the alpha 1 and alpha 2 domains during de novo synthesis.The Journal of Immunology, 1988
- A high molecular weight protease in the cytosol of rat liver. I. Purification, enzymological properties, and tissue distribution.Journal of Biological Chemistry, 1986
- Attachment of an anti-receptor antibody to non-target cells renders them susceptible to lysis by a clone of cytotoxic T lymphocytes.Proceedings of the National Academy of Sciences, 1984
- Immunoprecipitation of cell surface structures of cloned cytotoxic T lymphocytes by clone-specific antisera.Proceedings of the National Academy of Sciences, 1984